Future Events connected with Project:
Year 2021
Seminar Speaker Series’ of project StruBioMol
Dr. Jacob BAUER
Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia
Normal Mode Analysis and Its Application to Protein Structure Studies
14.01.2021 at 14:00
Abstract: Normal Mode Analysis (NMA) is a computational technique that can be used to calculate the flexible states available to a protein from a given equilibrium conformation. It produces results comparable to those from molecular dynamics simulation combined with principal components analysis but at a fraction of the computational cost. Although it has been used for certain applications in protein crystallography for many years, it is still uncommon to find it a part of the analysis step in a protein crystal structure study. Here, I will briefly describe the theory and most common applications of NMA. I will illustrate some of its strengths and limitations using a case study from the literature, and will discuss a potential application to single-molecule optical tweezers experiments
Zoom online video presentation: https://zoom.us/j/91905230005?pwd=anZ0aXlJandMTEpEcCtQUzJoMmxtZz09
Meeting-ID: 919 0523 0005
Kenncode: 997466
Host: Kristina Djinovic-Carugo
Past Events connected with Project:
Year 2020
Dr. Kvido Stříšovský
Membrane protein quality control and intramembrane proteolysis
06.10.2020 at 13:00
Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia
Zoom online video presentation: https://us02web.zoom.us/j/202574764?pwd=QjlDQ2tiU09RSUJKQkZVQUVQbWRZUT09
Thomas Gohl, PhD. and Mayank Aggarwal, PhD.
FORMULATRIX Workflow solution
29.09.2020 at 14:00
Program:
- Quick Introduction (TG)
- Systems – Specific features of systems for high throughput automation (TG)
RI54 – Imager
Formulator – Screen Maker
NT8 – Drop Setter
- Rock Maker – Protein Crystallization Software (MA)
Grid Optimization
Random Optimization
Iterative Screen Optimization
MARCO – Machine Recognition of Crystallization Outcomes
WebEx online video presentation: the link will be send to the registered participants
Registration: by email to biochemiaumb@gmail.com
Choose the new gold standard in protein stability characterization
Piotr Wardega (NanoTemper)
Monday, July 20, 2020 at 1:00 PM in the lecture room of the IMB SAS
The lecture will include general information about differential scanning fluorimetry and principles of working with new nanoDSF instrument Prometheus from NanoTemper
Instructional lecture on Beckman ultracentrifuge
Thursday, July 9, 2020 at 1:00 PM in the lecture room of the IMB SAS
The training will include general procedures for ultracentrifuges, principles of working with rotors, test tubes and a practical demonstration of the newly installed hall ultracentrifuge Optima XPN
Dr. Cyril Bařinka
Structure-assisted design of ligands for prostate cancer imaging and therapy
31.03.2020 at 14:00
Lecture Hall at Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia
Year 2019
1st StruBioMol Workshop: Biophysical Characterisation and Crystallisation of Proteins (Registration)
The workshop will take place immediately after the Interreg SK-AT "CAPSID" workshop on An Introduction to Recombinant Protein Production, Purification, and Quality Assessment, which will be on the 3-4. December 2019 (https://capsid.vbcf.ac.at/events/), what bring a lot of synergies between both projects.
When: 05.12.2019 – 06.12.2019
Where: Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Campus Vienna Biocenter 5, A-1030, Vienna
Topics:
The workshop will provide an introduction into the biophysical characterisation and crystallisation of proteins. The covered biophysical techniques will be size exclusion chromatography, dynamic and static light scattering, differential scanning fluorimetry, and circular dichroism. The workshop is intended for Masters’ and PhD students, postdocs and other researchers in the Vienna-Bratislava cross-border region.
Contact:
In Case of any questions please contact interreg86@gmail.com
Speakers and tutors:
Kristina Djinovic Carugo, Max Perutz Labs, University of Vienna
Julius Kostan, Max Perutz Labs, University of Vienna
Joan Lopez Arolas, Max Perutz Labs, University of Vienna
Dalibor Milic, Max Perutz Labs, University of Vienna
Georg Mlynek, Max Perutz Labs, University of Vienna
Arthur Sedivy, Vienna BioCenter Core Facilities
Martin Puchinger, Vienna BioCenter Core Facilities
Vladena Bauerová, Slovak Academy of Sciences, Bratislava
Thursday 5th December
9:00 - 9:15 Welcome and Introduction
Kristina Djinovic Carugo, Eva Kutejova
9:15 - 10:30 Requirements on protein purity for biochemical and structural studies and size exclusion
chromatography
Kristina Djinovic Carugo
10:30 - 11:30 Static and dynamic light scattering
Arthur Sedivy
11:30 - 11:50 Coffee break
11:50 - 13:00 Assessment and optimisation of thermal stability with differential scanning fluorimetry
Georg Mlynek
13:00 - 14:00 Lunch
14:00 - 16:15 Practicals on biophysical methods
16:15 - 16:45 Coffee break
16:45 - 18:30 Practicals on biophysical methods
Friday 6th December
9:00 - 10:00 Crystallisation of macromolecules - part I
Kristina Djinovic Carugo
10:00 - 11:00 Crystallisation of macromolecules - part II
Kristina Djinovic Carugo
11:00 - 11:30 Coffee break
11:30 - 13:00 Circular dichroism
Vladena Bauerová
13:00 - 14:00 Lunch
14:00 - 16:15 Practicals on crystallisation and CD
16:15 - 16:45 Coffee break
16:45 - 18:30 Practicals on crystallisation and CD
[28.06.2019 Bratislava]
On June 28, Max Perutz Labs and IMB SAS partners met in Bratislava to launch the Interreg StruBioMol project. The project is aimed at increasing the international visibility of structural biology research in the Slovak-Austrian border region. Together with 23 strategic project partners, the University of Vienna - Max Perutz Labs in Vienna and the Institute of Molecular Biology of the Slovak Academy of Sciences in Bratislava have teamed up to improve scientific communication in the region and the international visibility of the structural biology and molecular biology research community.
StruBioMol is co-financed by the European Regional Development Fund (ERDF) under the Interreg V-A Slovakia-Austria program.
