Future Events connected with Project:

Year 2021

 

Seminar Speaker Series’ of project StruBioMol

 

DrJacob BAUER

Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia

Normal Mode Analysis and Its Application to Protein Structure Studies

14.01.2021 at 14:00

Abstract: Normal Mode Analysis (NMA) is a computational technique that can be used to calculate the flexible states available to a protein from a given equilibrium conformation. It produces results comparable to those from molecular dynamics simulation combined with principal components analysis but at a fraction of the computational cost. Although it has been used for certain applications in protein crystallography for many years, it is still uncommon to find it a part of the analysis step in a protein crystal structure study. Here, I will briefly describe the theory and most common applications of NMA. I will illustrate some of its strengths and limitations using a case study from the literature, and will discuss a potential application to single-molecule optical tweezers experiments 

 

Zoom online video presentation: https://zoom.us/j/91905230005?pwd=anZ0aXlJandMTEpEcCtQUzJoMmxtZz09

Meeting-ID: 919 0523 0005

Kenncode: 997466

Host: Kristina Djinovic-Carugo

https://www.maxperutzlabs.ac.at/news/lectures/l/normal-mode-analysis-and-its-application-to-protein-structure-studies-1003

 

 

Past Events connected with Project:

Year 2020

Dr. Kvido Stříšovský

Membrane protein quality control and intramembrane proteolysis

06.10.2020 at 13:00

Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia

Zoom online video presentation: https://us02web.zoom.us/j/202574764?pwd=QjlDQ2tiU09RSUJKQkZVQUVQbWRZUT09

 


Thomas Gohl, PhD. and Mayank Aggarwal, PhD.

FORMULATRIX Workflow solution

29.09.2020 at 14:00

Program: 

- Quick Introduction (TG)

- Systems – Specific features of systems for high throughput automation (TG)

               RI54 –                     Imager

               Formulator –           Screen Maker

               NT8 –                       Drop Setter

- Rock Maker – Protein Crystallization Software (MA)

               Grid Optimization

               Random Optimization

               Iterative Screen Optimization

               MARCO – Machine Recognition of Crystallization Outcomes

WebEx online video presentation: the link will be send to the registered participants

Registration: by email to biochemiaumb@gmail.com

 


Choose the new gold standard in protein stability characterization

Piotr Wardega (NanoTemper)

Monday, July 20, 2020 at 1:00 PM in the lecture room of the IMB SAS

The lecture will include general information about differential scanning fluorimetry and principles of working with new nanoDSF instrument Prometheus from NanoTemper

 


Instructional lecture on Beckman ultracentrifuge

Thursday, July 9, 2020 at 1:00 PM in the lecture room of the IMB SAS

 

The training will include general procedures for ultracentrifuges, principles of working with rotors, test tubes and a practical demonstration of the newly installed hall ultracentrifuge Optima XPN

 


Dr. Cyril Bařinka

Structure-assisted design of ligands for prostate cancer imaging and therapy

31.03.2020 at 14:00

Lecture Hall at Institute of Molecular Biology SAS, Dúbravská cesta 21, Bratislava, Slovakia

 


Year 2019

1st StruBioMol WorkshopBiophysical Characterisation and Crystallisation of Proteins (Registration)

The workshop will take place immediately after the Interreg SK-AT "CAPSID" workshop on An Introduction to Recombinant Protein Production, Purification, and Quality Assessment, which will be on the 3-4. December 2019 (https://capsid.vbcf.ac.at/events/), what bring a lot of synergies between both projects.

When: 05.12.2019 – 06.12.2019

Where: Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Campus Vienna Biocenter 5, A-1030, Vienna

Topics:

The workshop will provide an introduction into the biophysical characterisation and crystallisation of proteins. The covered biophysical techniques will be size exclusion chromatography, dynamic and static light scattering, differential scanning fluorimetry, and circular dichroism. The workshop is intended for Masters’ and PhD students, postdocs and other researchers in the Vienna-Bratislava cross-border region.

Contact:

In Case of any questions please contact interreg86@gmail.com

Speakers and tutors:

Kristina Djinovic Carugo, Max Perutz Labs, University of Vienna

Julius Kostan, Max Perutz Labs, University of Vienna

Joan Lopez Arolas, Max Perutz Labs, University of Vienna

Dalibor Milic, Max Perutz Labs, University of Vienna

Georg Mlynek, Max Perutz Labs, University of Vienna

Arthur Sedivy, Vienna BioCenter Core Facilities 

Martin Puchinger, Vienna BioCenter Core Facilities

Vladena Bauerová, Slovak Academy of Sciences, Bratislava

Program:

Thursday 5th December

9:00 - 9:15      Welcome and Introduction

Kristina Djinovic Carugo, Eva Kutejova

 

9:15 - 10:30      Requirements on protein purity for biochemical and structural studies and size exclusion

chromatography

Kristina Djinovic Carugo

10:30 - 11:30    Static and dynamic light scattering

Arthur Sedivy

11:30 - 11:50     Coffee break

11:50 - 13:00    Assessment and optimisation of thermal stability with differential scanning fluorimetry

Georg Mlynek

13:00 - 14:00    Lunch

14:00 -  16:15   Practicals on biophysical methods

16:15 - 16:45   Coffee break

16:45 - 18:30   Practicals on biophysical methods

 

Friday 6th December

9:00 - 10:00     Crystallisation of macromolecules - part I

Kristina Djinovic Carugo

10:00 - 11:00    Crystallisation of macromolecules - part II

Kristina Djinovic Carugo

11:00 - 11:30    Coffee break

11:30 - 13:00    Circular dichroism

Vladena Bauerová

13:00 - 14:00    Lunch

14:00 -  16:15   Practicals on crystallisation and CD

16:15 - 16:45   Coffee break

16:45 - 18:30   Practicals on crystallisation and CD

 

[28.06.2019 Bratislava]

On June 28, Max Perutz Labs and IMB SAS partners met in Bratislava to launch the Interreg StruBioMol project. The project is aimed at increasing the international visibility of structural biology research in the Slovak-Austrian border region. Together with 23 strategic project partners, the University of Vienna - Max Perutz Labs in Vienna and the Institute of Molecular Biology of the Slovak Academy of Sciences in Bratislava have teamed up to improve scientific communication in the region and the international visibility of the structural biology and molecular biology research community.


StruBioMol is co-financed by the European Regional Development Fund (ERDF) under the Interreg V-A Slovakia-Austria program.