Chronic, non-healing wounds are the major cause of morbidity in elderly population. These non-healing wounds are often characterised by an excessive, and detrimental inflammatory response accompanied by bacterial colonization. Prevalence of antibiotic-resistant bacteria and bacterial biofilms in wounds is a significant problem in wound care and therefore, it urgently needs to find alternative biomedical strategies. One of these strategies represents apitherapy ? the medical use of honeybee products including medical-grade honeys, propolis and royal jelly in the treatment of chronic and infected wounds.
Laboratory of apidology and apitherapy focus on the apimedical research and characterisation the bee products-induced mechanisms of wound healing and tissue remodelling. Besides the direct antimicrobial and antibiofilm effect, bee products possess the anti-inflammatory and immunomodulatory effects. Recently, the multifunctional immunomodulatory properties of bee products on cells involved in wound healing such as monocytes, keratinocytes and fibroblasts have attracted much attention. Our laboratory together with Department of microbiology at Slovak medical university also participates on translational research through the performing in vivo experiments on an animal model or human clinical trials using a Slovak honeydew honey in wound care. An important part of our research is the quality and authenticity of honeybee products with reference to the content of individual biologically active components where we focus on the development of new qualitative/quantitative tests. A naturally increased content of bee secreted biologically active compounds in honey and royal jelly may promote to protect bee and its offspring and to wider therapeutic use of these products.
A complete, molecular understanding of the workings of any organism requires an investigation of the structure - function relationship for all proteins in the cell. Bacillus subtilis is an internationally-recognized model organism, whose physiology, biochemistry and genetics has been studied for many years. Department of Microbial Genetics projects are oriented toward studying the proteins involved in basic processes in Bacillus subtilis as cell division, sporulation and programmed cell death. Asymmetric cell division is fundamental to the development not only of multicellular organisms but also to rod-shaped bacteria that undergo a specific differentiation process called sporulation. The target proteins are especially Spo0A, SpoIIE, DivIVA, MinC, MinD, RodZ and their complexes. In case of programmed cell death we concentrate on biochemical, genetic and structural studies of toxin-antitoxin, SpoIISA-SpoIISB system. These proteins were firstly identified as part of sporulation process in Bacillus subtilis. Genetic, biochemical experiments and especially tertiary structure of above mentioned proteins would be a great contribution for our understanding of basic bacterial cell processes.
Department is also involved in studies of protein-protein interactions of sporulation specific Cot proteins which are able to form highly ordered nano-scale layers. The aim of these studies is to develop new tools and nano-array based systems to form layers from protein molecules up to the scale of nano-particles. These technologies should be tested in a few trial applications for preparation of nano-materials as well as in new bio-nanotechnologies processes.
International scientific co-operation
- Biological Physical Sciences Institute, University of York, York, United Kingdom
- Changchun Institute of Applied Chemistry,
Chinese Academy of Sciences, Changchun, China
- Department Biochemistry & Biotechnology, University of Thessaly, Larissa, Greece
- Department of Chemistry, University of York, York, United Kingdom
- Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland
- DISIT - Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Alessandria, Italy
- EPFL, Lausanne, Switzerland
- Royal Holloway and Bedford New College, London, United Kingdom
- XFEL, Hamburg, Germany